发明名称 |
Method of producing partially purified extracellular metabolite products from <i>Bacillus coagulans </i>and biological applications thereof |
摘要 |
Method of extracting a partially purified extracellular metabolite composition from Bacillus coagulans MTCC 5856 and its antimicrobial effects thereof are described. |
申请公布号 |
US9596861(B2) |
申请公布日期 |
2017.03.21 |
申请号 |
US201414576411 |
申请日期 |
2014.12.19 |
申请人 |
SAMI LABS LIMITED |
发明人 |
Majeed Muhammed;Nagabhushanam Kalyanam;Arumugam Sivakumar;Ali Furqan |
分类号 |
A61K35/00;A01N63/02;C12N1/20;C12R1/07;A01N31/08;A01N37/12;C12R1/085;C12R1/19;C12R1/385;C12R1/42;C12R1/445;C12R1/45;C12R1/46 |
主分类号 |
A61K35/00 |
代理机构 |
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代理人 |
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主权项 |
1. A purification method for producing partially purified extracellular metabolite preparation from the probiotic bacterial strain Bacillus coagulans strain MTCC 5856), said purification method comprising the steps of:
a) Inoculating a culture of Bacillus coagulans MTCC 5856 into 1.0 liter of Glucose Yeast Extract Acetate broth medium or MRS broth containing 0.5% Tween 80 or Corn steep powder media to initiate bacterial fermentation; b) Allowing the bacterial fermentation in the inoculated medium of step a to proceed for 24-48 h at 37° C. with 120 rpm; c) Centrifuging the fermentation broth of step b at 4000-7000 rpm and collecting supernatant; d) Concentrating supernatants 10 fold by using rotary evaporator at 50° C. of step c; e) Adding 150 ml of chilled acetone drop by drop to 100 ml of tenfold concentrated supernatants of step d, followed by mixing to form supernatants-acetone mixture; f) Incubating the supernatants-acetone mixture of step e at 0° C. for 30 minutes followed by centrifuging at 7000-8000 rpm to collect a pellet; g) Discarding the pellet obtained in step f and collecting 60% (v/v) acetone saturated supernatant (˜200 ml); h) Concentrating the acetone saturated supernatant in step g to 50 ml by rotary evaporator; i) Adjusting the pH of the supernatant of step h to 5.0 by using 4N HCl, filtered (0.22 micron; Millex, Millipore, India) and stored at −20° C. till further use; j) Freeze drying the supernatant of step I to obtain partially purified extracellular metabolite preparation from the probiotic bacterial strain Bacillus coagulans MTCC 5856. |
地址 |
Bangalore IN |