SINGLE-GUIDE RNA (SGRNA) WITH IMPROVED KNOCKOUT EFFICIENCY

摘要

The present invention includes a modified single-guide RNA (sgRNA) template with improved knockout efficiency specific for a target gene comprising an sgRNA construct comprising at least one of a mutated duplex region wherein a length of the duplex region is extended, or a mutated poly T region at the beginning of the duplex region, wherein the sgRNA has a greater target gene knockout efficiency in cells.