Peptide biomarkers of cardiovascular disease

摘要

The presently-disclosed subject matter provides methods for diagnosing a cardiovascular disease in a subject by determining an amount of one or more peptide biomarkers disclosed herein in a biological sample from the subject. The presently-disclosed subject matter further provides methods for determining treatment efficacy and/or progression of a cardiovascular disease in a subject by measuring amounts of one or more of the biomarkers in a biological sample from the subject. The presently-disclosed subject matter also provides antibodies and kits for measuring the biomarkers.

主权项

1. A method for identifying a biomarker peptide for diagnosing a cardiovascular disease, comprising:
(a) obtaining a biological sample from a subject having or at risk of having a cardiovascular disease; (b) fractionating the biological sample to obtain an unbound fraction having the biomarker peptide free from albumin and immunoglobulin present in the biological sample and a bound fraction having the biomarker peptide bound to albumin or immunoglobulin present in the biological sample; and (c) determining an amount of the biomarker peptide in the unbound fraction, the bound fraction, or both the unbound fraction and the bound fraction, the biomarker peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOS: 8-30.