CONDITIONED CELL CULTURE MEDIUM COMPOSITIONS AND METHODS OF USE

摘要

Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, or formulated with a salve or ointment for topical applications. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.

主权项

1. A conditioned medium for making a composition suitable for topical application, the conditioned medium made by a process comprising the steps of:
(a) inoculating fetal fibroblasts or embryonic stem cells onto a substrate in a nutrient medium; (b) culturing the fetal fibroblasts or embryonic stem cells in a two dimensional culture so that the fetal fibroblasts or embryonic stem cells secrete a growth factor and a collagen into the nutrient medium, until the nutrient medium becomes a conditioned medium; and (c) removing the conditioned medium from the cultured cells.