ANTIGEN DETECTION USING PHOTOCLEAVABLE LABELS

摘要

Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.

主权项

1. A method for detecting the presence of at least the first antigen on or in a sample comprising a first antigen-binding complex capable of specifically binding the first antigen, the method comprising detecting the presence of a photocleavable label in the sample, wherein the photocleavable label is conjugated to the first antigen-binding complex through a functional linker, wherein the first antigen-binding complex is non-covalently bound to the first antigen forming an antigen-bound antigen-binding complex of formula (I): wherein the presence of the photocleavable label is indicative of the presence of the first antigen.