Micro-Tube Particles for Microfluidic Assays and Methods of Manufacture

摘要

A method for preparing a plurality of micro-length tubular flow elements for use in a fluid assay, each element having interior and exterior surfaces and having at least one axially-extending flow passage through its interior, includes aggressively agitating the flow elements in a solution of assay capture agent to impart disrupting shear forces on the exterior surface of the elements, the shear forces causing the axially-extending external surfaces of the flow elements to be free of assay capture agent, while at least a portion of the interior surface of the flow elements not experiencing such disruptive shear forces and carrying deposits of the assay capture agent.

主权项

1. A method for preparing a plurality of transparent, micro-length tubular flow elements for use in a fluid assay, each flow element having interior and exterior surfaces and having at least one axially-extending flow passage through its interior, comprising:
suspending the flow elements in a solution of assay capture agent, the capture agent to be used during the assay to capture an analyte; and aggressively agitating the flow elements in the solution of capture agent to impart disrupting shear forces on the exterior surface of the elements, thereby causing the axially-extending external surfaces of the flow elements to be free of the assay capture agent, while at least a portion of the interior surfaces of the flow elements carrying deposits of the assay capture agent.