EXPRESSION OF TRIPLE-HELICAL COLLAGEN-LIKE PRODUCTS IN E.COLI

摘要

Recombinant bacterial triple-helical collagen-like proteins comprising two or more repetitive sequences of Gly-Xaa-Yaa yielding high-stability polymeric constructs without the need for post-translational modifications and which may incorporate one or more functional domains of biological or structural importance. The polymers are capable of high-yield production for a variety of applications

主权项

1. A recombinant bacterial collagen-like protein structure which is stable at temperatures between 35° C. and 40° C. in either its native form or after chemical crosslinking, comprising a formula:
[(Gly-Xaa-Yaa)m-(insert)n]p where m is between about 1 to 200 and (Gly-Xaa-Yaa)m represents a triple helical domain with Xaa and Yaa being independently any natural or unnatural imino or amino acid for each repeat unit, wherein the insert is comprised of about 1 to 50 of any imino or amino acids, with n being 0 or 1, and p being any number from about 2 to about 10 wherein the value of n is unique for each repeat and at least one insert is provided in the collagen-like protein.