Single-use biomass sensing device, method for producing this device and single-use bioreactor incorporating this sensor

摘要

A single-use bioreactor or container including an enclosure provided for containing a biological medium, and at least one device for making at least one impedance measurement in a physical, physico-chemical and/or biological process in this medium. The sensing device is fixed through a wall of the enclosure in such a way as to be an integral part thereof and includes a detection part in direct contact with an inside of the bioreactor or container and a connection part extending towards an outside of the enclosure.

主权项

1. A single-use bioreactor or container comprising:
an enclosure provided for containing a biological medium; a control device; and at least one single-use sensing device for making at least one impedance measurement in a physical, physico-chemical and/or biological process in said biological medium, said sensing device including a detection part with at least four electrodes for impedance measurements on a detection face arranged inside said single-use bioreactor or container so as to be in direct contact with said biological medium, said at least four electrodes being coupled to said control device and designed to avoid effects of double-layer polarization, and said detection part being fixed by gluing or by welding to an inner face of a wall of said enclosure so as to be an integral part thereof; wherein said control device is configured to determine parameters of a β dispersion curve from the impedance measurements of said at least four electrodes.