Resistive sensor based on conductivity change of conductive polymer and measuring method thereof

摘要

A resistive sensor for an analyte comprises a substrate, a conductive polymer layer and an oxidase layer. Hydrogen peroxide is produced via the reaction between analyte and oxidase when a liquid sample is applied to the sensor of the present invention. The produced hydrogen peroxide can oxidize peroxidase, which can be reduced by oxidizing the conductive polymer, thus resulting in decreased conductivity of the conductive polymer for determining the analyte concentration in the liquid sample. The present invention may be used for developing miniaturized and disposable electronic microsensors with high sensitivity and fast response, which can detect analyte level in typical physiological environment for routine monitoring.

主权项

1. A resistive sensor for an analyte, comprising:
a substrate; a conductive polymer layer configured on the substrate and made of polyaniline or derivatives thereof, wherein a peroxidase is configured on the conductive polymer layer and used for oxidizing the conductive polymer layer so that a conductivity of the conductive polymer layer is decreased after applying an analyte, wherein a conductivity change of the conductive polymer layer is related to a concentration of the analyte; and an oxidase layer configured on the conductive polymer layer and provided with an oxidase configured thereon, wherein the oxidase is separated from the peroxidase and used for oxidizing the analyte to generate a hydrogen peroxide so that the peroxidase is oxidized by the hydrogen peroxide.