Compositions, processes and algorithms for microbial detection

摘要

Processes for identifying whether any parasite or other organism is present in a host comprising: a. scanning for non-host signatures, b. scanning for one-error-removed non-host signatures; c. scanning for N-error removed non-host signatures; where N is selected to give the desired statistical certainty of the presence or absence of any parasite in the host. Algorithms useful for such detections and listings of specific signatures” (sequences or subsequences) for identifying specific microorganisms are also both provided.

主权项

1. A process for identifying whether a specific target organism (including viruses) is present in a sample, the process comprising in combination:
a. identifying at least one nucleic acid sequence (n-mer) characteristic of the target organism; b. identifying at least one nucleic acid which may be present in the sample and is characteristic of one or more other (nontarget) organisms which may be present in the sample; c. identifying nucleic acid sequence(s) of the organism or virus differing in at least N positions (N mismatches, where N is 1-10) from the nucleic acid sequence(s) of one or more other (non target) organisms which may be present in the sample; and d. determining the presence in the sample of one or more sequence(s) from step a which do not meet the criteria of step b and do meet those of step c, whereby the presence of such sequences indicates the presence of the target organism.