Methods for reducing intraocular pressure by administering a soluble hyaluronidase glycoprotein (sHASEGP)

摘要

Provided are methods for reducing intraocular pressure in the eye of a subject by administering neutral active soluble active hyaluronidase glycoproteins (sHASEGPs) and modified forms thereof to the eye. The sHASEGPs are soluble forms of human PH20 hyaluronidase. Also provided are the sHASEGPs, methods of manufacture, and uses thereof. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described. The sHASEGPs include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain.

主权项

1. A method for reducing intraocular pressure in the eye of a subject, comprising administering a composition comprising a hyaluronidase polypeptide to the eye, wherein:
the hyaluronidase polypeptide contains at least one sugar moiety that is covalently attached to an asparagine residue of the hyaluronidase polypeptide; the hyaluronidase polypeptide is catalytically active; the hyaluronidase polypeptide is secreted; and the hyaluronidase polypeptide consists of a sequence of amino acids that has at least 98% amino acid sequence identity with the sequence of amino acids set forth as amino acid residues 36-483 of SEQ ID NO:1.