Optimizing operating binding capacity for a multiple column chromatography process

摘要

The invention relates to multiple column chromatography (MCC). MCC is complicated and there is a need for improved MCC process development, The invention provides methods for optimizing the operating binding capacity for a multiple column chromatography (MCC) process. In particular, the invention provides methods for determining an optimum operating binding capacity for the MCC process. Product Feed Column A Coimn B (X) amount bound to coumn A ( Y amount bound to column B 2 -Column A Inlet concentration Column A Outlet concentration w Column B Outlet concentration C0,4 0 2C) 40 60 0 10 Time. (min) Start of product breakthrough at column B outlet RTt Coclmtm A VRT= f R=2a L Fv RT= C C umr/BV -Gv 4IU