METHOD AND COMPOSITIONS FOR CONTROLLING INSECT PESTS ON PLANTS BY SILENCING GENES OF THE CHITIN SYNTHASE AND VITELLOGENIN FAMILY, AND ALTERNATIVELY BY EXPRESSION OF A CRY TOXIN GENE

摘要

The present invention relates to the control of pest infestation by inhibiting or reducing the expression of genes of the family of chitin synthase and of vitellogenin, as well as through the expression of the toxin Cry8ka5. The invention further provides method and compositions for controlling pests, by feeding the pest with one or more double-stranded RNA molecules provided by the present invention, as well as double-stranded RNA molecule provided by the present invention, as well as through the action of the toxin Cry8ka5 on the target insect. The invention further describes a method of obtaining transgenic plants that express double-stranded RNA molecules and the toxin protein Cry8ka5. The present invention is preferably used for cotton plants.

主权项

1. A gene construct characterized by comprising:
(i) a promoter functional in plant; (ii) a sense fragment substantially similar to the SEQ ID NO: 1; (iii) a sense fragment substantially similar to the SEQ ID NO: 2; (iv) a spacer sequence; (v) an antisense fragment substantially similar to the SEQ ID NO: 3; (vi) an antisense fragment substantially similar to the SEQ ID NO: 4; and (vii) a terminator functional in plant.