GENE TARGETING USING MUTANT AGROBACTERIUM STRAINS

摘要

The present invention provides methods and compositions for enhancing the efficiency of Agrobacterium-mediated transformation of host cells, such as plant cells, wherein the host cells overexpress LSH10 protein. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a DNA of interest to be inserted into a plant genome at a homologous position.

主权项

1. A transgenic plant or part thereof transformed with a polynucleotide sequence encoding a LSH10 polypeptide, operably linked to a heterologous promoter functional in the plant or part thereof, wherein the plant or part thereof exhibits increased transformability by Agrobacterium-mediated transformation relative to a plant or part thereof lacking the polynucleotide sequence.