VECTORS AND STRAINS FOR PRODUCING MYRCENE AND METHOD OF PRODUCING MYRCENE USING THE SAME

摘要

Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.

主权项

1. A transformed Escherichia coli strain transformed with a first vector and a second vector,
the first vector comprising, in sequence, a chloramphenicol resistance gene as a selection marker; a p15A replication origin as a replication origin; a lacUV5 promoter; a first domain comprising a gene encoding an enzyme which produces mevalonate from acetyl-CoA; and a second domain comprising a gene encoding an enzyme which produces dimethylallyl pyrophosphate (DMAPP) from mevalonate, and the second vector comprising, in sequence, an ampicillin resistance gene as a selection marker; a ColE1 replication origin as a replication origin; a trc promoter; and a gene encoding an enzyme which is capable of producing myrcene from geranyl pyrophosphate (GPP).