发明名称 |
METHOD FOR SELECTIVELY MANUFACTURING ANTIBODY-DRUG CONJUGATE |
摘要 |
A method for manufacturing an antibody-drug conjugate composition comprising (i) a step for reacting an antibody with a reducing agent in a buffer solution to reduce the interchain disulfides, and (ii) a step for reacting a drug-linker intermediate with the antibody having a thiol group obtained in step (i), the manufacturing method being characterized in that: the reaction temperature in step (i) is -10°C to 10°C; the average number of coupled drugs in the manufactured antibody-drug conjugate composition is 3.5 to 4.5; and the proportion of antibody-drug conjugates in which four drug-linkers are coupled to a heavy-light interchain thiol is 50% or higher. An antibody-drug conjugate composition in which the average number of coupled drugs is 3.5 to 4.5, and the proportion of antibody-drug conjugates in which four drug-linkers are coupled to a heavy-light interchain thiol is 50% or higher. |
申请公布号 |
WO2017002776(A1) |
申请公布日期 |
2017.01.05 |
申请号 |
WO2016JP69068 |
申请日期 |
2016.06.28 |
申请人 |
DAIICHI SANKYO COMPANY,LIMITED |
发明人 |
NOGUCHI, Shigeru;SAKURAI, Ken;OKAJIMA, Daisuke |
分类号 |
C07K16/00;A61K31/40;A61K31/4745;A61K39/395;A61K45/00;A61P35/00;C07K16/28;C07K16/32 |
主分类号 |
C07K16/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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