METHOD FOR MOLECULAR DIAGNOSTICS FOR ENRICHING A NUCLEIC ACID FROM A BIOLOGICAL SAMPLE

摘要

The invention relates to a method for molecular diagnostics, for enriching nucleic acids from phagocytised cells of a first cell type from a biological sample of a patient, the biological sample comprising cells of a phagocytising cell type, which cells each phagocytise a cell of the first cell type, in which at least a portion of the phagocytising cells of the biological sample is marked (1) by antibodies specific to the phagocytising cells, the antibodies each being coupled to magnetic markers, then the marked phagocytising cells are separated out (3) of the biological sample by means of a magnetic field applied by a cell separation device, as a result of which the marked phagocytising cells are enriched and thus the nucleic acids of the phagocytised cells are enriched, in order to provide a fast and simple routine for point-of-care testing, in particular for the diagnosis of sepsis.