Cell lines expressing ENaC and methods using them

摘要

Cell lines that stably express ENaC and methods for using those cell lines are disclosed herein. The invention includes cell lines that express various subunit combinations and various proteolyzed isoforms of ENaC and techniques for creating cell lines. The ENaC-expressing cell lines are highly sensitive, physiologically relevant and produce consistent results.

主权项

1. A stably transfected engineered cell line, or a cell from the cell line, stably expressing:
a) an epithelial sodium channel (ENaC) alpha or delta subunit, b) an ENaC beta subunit, and c) an ENaC gamma subunit; wherein the cell line, or the cell from the cell line, maintains, for at least one week, the ability to produce a Z′ value of at least 0.3 in a membrane potential dye assay using NaCI as an agonist.