发明名称 |
Method of producing pancreatic hormone-producing cells |
摘要 |
The purpose of the present invention is to provide a method of producing pancreatic hormone-producing cells that have a form more similar to that of normally developed pancreatic cells from stem cells. The method of producing pancreatic hormone-producing cells is characterized by subjecting stem cells to the following steps (1)-(6): (1) a step for culturing stem cells in a culture medium that includes a Rho kinase inhibitor; (2) a step for culturing the cells obtained in step (1) in a culture medium including a GSK3 inhibitor; (3) a step for culturing the cells obtained in step (2) in a culture medium including a GSK3 inhibitor and an agent that activates activin receptor-like kinases 4 and 7; (4) a step for forming a cell aggregate of the cells obtained in step (3) and culturing said cell aggregate in a floating state within a culture medium; (5) a step for culturing the cells obtained in step (4) in a culture medium including a retinoic acid receptor agonist, an AMP-activated protein kinase and/or an activin receptor-like kinase 2, 3 and 6 inhibitor, an activin receptor-like kinase 4, 5 and 7 inhibitor and a cell growth factor; and (6) a step for culturing the cells obtained in step (5) in a culture medium. |
申请公布号 |
AU2011290123(B2) |
申请公布日期 |
2016.11.10 |
申请号 |
AU20110290123 |
申请日期 |
2011.08.08 |
申请人 |
Takeda Pharmaceutical Company Limited |
发明人 |
Hosoya, Masaki;Shoji, Masanobu |
分类号 |
C12N5/0735;A61K35/12;A61P3/10;C12N5/10;C12Q1/02 |
主分类号 |
C12N5/0735 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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