| 摘要 |
The present invention provides a method of maintaining and amplifying pluripotent stem cells, including repeating the following steps:
(i) suspension culturing pluripotent stem cells until cell aggregates have an average diameter of about 200 - about 300 µm,
(ii) fragmenting the cell aggregates obtained by step (i) into cell aggregates having a uniform average diameter of about 80 - about 120 µm. In step (i), a suitable viscosity is conferred to the medium to prevent movement of floating cell aggregates and adhesion and fusion of cell aggregates. In step (ii), the cell aggregates are mechanically fragmented into smaller uniform cell aggregates by passing the cell suspension through a mesh. |
