发明名称 |
Use of a laminin for differentiating pluripotent cells into hepatocyte lineage cells |
摘要 |
The invention relates to the use of a laminin (LN) as a matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human pluripotent cells, (ii) culturing the population on a support coated with a laminin in a endoderm induction medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a support coated with a laminin in a hepatic induction medium to produce a population of human hepatoblasts-like cells, and (iv) optionally culturing said population of human hepatoblasts-like cells on a support coated with a laminin in a hepatic maturation medium to produce a population of human fetal hepatocyte-like cells. The invention further relates to a population of human hepatoblasts-like cells or human fetal hepatocyte-like cells obtained by the method of the invention. The invention further relates to a population of human hepatoblasts-like cells expressing HNF4± and not expressing or substantially not expressing AFP for use in a method of treatment of the human body. |
申请公布号 |
EP3059307(A1) |
申请公布日期 |
2016.08.24 |
申请号 |
EP20150305265 |
申请日期 |
2015.02.20 |
申请人 |
INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE);UNIVERSITÉ DE NANTES |
发明人 |
NGUYEN, TUAN HUY;FOURRIER, ANGÉLIQUE |
分类号 |
C12N5/071 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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