METHOD FOR MANUFACTURING SMALL-DIAMETER COLUMN FOR CHROMATOGRAPHY

摘要

Packing a column with a chromatography medium at prescribed compression factor , and then passing a mobile phase through the column packed with the chromatography medium while switching the mobile phase flow direction two or more times. According to the present invention, a small-diameter chromatography column packed uniformly and densely as well as a large-diameter column can be reproducibly prepared. The small-diameter chromatography column prepared in this way has the asymmetry factor of not less than 0.80 and not more than 1.80.