| 摘要 |
The invention relates to a process for selecting microorganisms consortia intended to treat plant material. The claimed process comprises the steps of: depositing a detecting film on a single-well plate; adding a minimal medium on the detecting film, i. e. 1 g to 100 ml hydro-jellified cellulose material with a triblock copolymer; inoculating it with 12 isolates for testing, randomly distributed in 8 repetitions; incubating the plate for 60...120 h, while sampling the image of the detecting film and microorganism colonies, respectively, each 12 h; making, in the same conditions, a witness plate, where each colony from the isolates tested is developed separately, by inoculating the 12 isolates in 8 repetitions, on a 96-well plate, with the lower part open, arranged over the detecting film and which contains in each well the same minimal hydro-jellified medium; analyzing the sampled images; comparing the results from the sample with the isolates developed in reciprocal interaction in the single-well plate with those from the witness plate, in which each isolate for testing was separately developed; identifying the reciprocally interacting microorganisms in development as antagonism or stimulation, and in the production of compounds of interest in the lignocellulose material. |
