发明名称 |
METHOD AND APPARATUS FOR DETECTING AND COUNTING PLATELETS INDIVIDUALLY AND IN AGGREGATE CLUMPS |
摘要 |
A method for enumerating platelets within a blood sample is provided. The method includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; 2) admixing a colorant with the sample, which colorant is operative to cause the platelets to fluoresce upon exposure to one or more predetermined first wavelengths of light; 3) illuminating at least a portion of the sample containing the platelets at the first wavelengths; 4) imaging the sample, including producing image signals indicative of fluorescent emissions from the platelets, which fluorescent emissions have an intensity; 5) identifying the platelets by their fluorescent emissions, using the image signals; 6) determining an average fluorescent emission intensity value for the individual platelets identified within the sample; 7) identifying clumps of platelets within the sample using one or more of their fluorescent emissions, area, shape, and granularity; and 8) enumerating platelets within each platelet clump using the average fluorescent emission intensity value determined for the individual platelets within the sample. |
申请公布号 |
EP3026433(A1) |
申请公布日期 |
2016.06.01 |
申请号 |
EP20160150962 |
申请日期 |
2009.03.20 |
申请人 |
ABBOTT POINT OF CARE, INC. |
发明人 |
WARDLAW, STEPHEN C.;LEVINE, ROBERT A.;LALPURIA, NITEN V. |
分类号 |
G01N33/50;G01N15/02;G01N15/14;G01N33/49;G01N33/58;G01N33/80 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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