HCV genotype 6 replicons

摘要

Replicons of genotype 6 hepatitis C virus (HCV) are provided. These replicons contain adaptive mutations giving rise to the HCV's capability to replicate in vitro. Methods of preparing genotype 6 replicons and methods of using these replicons to screen antiviral agents are also provided.

主权项

1. A genotype 6 hepatitis C viral (HCV) RNA construct comprising a 5′UTR, sequences encoding NS4A, NS5A, and one or more of NS3, NS4B, or NS5B, and a 3′UTR, wherein the NS4A comprises a K34R mutation and the NS5A comprises an S232I mutation, each as compared to a wildtype genotype 6 HCV.