| 摘要 |
The present invention relates to a quantitative analysis method for nucleic acids in a sample, the method applying a spiking-in neighbor genome-coupled competitive PCR amplicon sequencing (SiNG-PCRseq) method in which a neighbor genome is mixed with a sample to be quantified, and then co-amplification is carried out by using similarities between neighboring sequences. Specifically, the SiNG-PCRseq method of the present invention can provide a standardized and relative DNA quantitative value under various experimental conditions including a variable element such as a cell line and a polymerase, and when compared with a RNA-seq method to be used as a conventional method for quantitative analysis of nucleic acids, the provided standardized DNA quantitative value accurately and conveniently provides a relative quantitative value with respect to nucleic acids in a sample irrespective of the experimental conditions or the type of sample, and thus the SiNG-PCRseq method of the present invention is useful for a standardized quantitative analysis method for nucleic acids in a sample. |
