Compositions and methods for detection of microorganisms of the <i>Mycobacterium avium </i>complex excluding <i>Mycobacterium avium paratuberculosis </i>

摘要

Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of a non-Mycobacterium avium subsp. paratuberculosis (non-MAP) organism, wherein a non-MAP organism is a Mycobacterium avium complex (MAC) organism that does not belong to the Mycobacterium avium subsp. paratuberculosis (MAP) organism, from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample. Exemplary non-MAP organisms including M. avium subsp. avium (MAA), M. avium subsp. hominissuis (MAH), and M. avium subsp. silvaticum (MAS) can be detected by the present compositions, kits and methods.

主权项

1. A method for differentially detecting a non-Mycobacterium avium subsp. paratuberculosis (non-MAP) Mycobacterium Avium Complex (MAC) organism selected from the group consisting of Mycobacterium avium subsp. Avium (MAA), Mycobacterium avium subsp silvaticum (MAS), and Mycobacterium subsp hominissuis (MAH), from a Mycobacterium avium subsp. paratuberculosis (MAP) organism comprising:
amplifying from a sample at least one non-MAP-specific target nucleic acid selected from the group consisting of SEQ ID NO:1, SEQ ID. NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and full complements thereof, comprising contacting nucleic acids from the sample with at least one primer set that can hybridize to and amplify the at least one non-MAP-specific target nucleic acid, under conditions suitable for amplification; and detecting an amplified nucleic acid, wherein detecting the amplified nucleic acid is indicative of the presence of a non-MAP MAC organism in the sample.