NON-CALORIC SWEETENERS AND METHODS FOR SYNTHESIZING

摘要

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebaudioside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).

主权项

1. A method for synthesizing rebaudioside E from rebaudioside KA, the method comprising:
preparing a reaction mixture comprising rebaudioside KA, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and a UDP-glycosyltransferase selected from the group consisting of HV1, EUGT11 and a UDP-glycosyltransferase fusion enzyme; and incubating the reaction mixture for a sufficient time to produce rebaudioside E, wherein a glucose is covalently coupled to the C2′ 13-O-glucose of rebaudioside KA to produce rebaudioside E.