摘要 |
The present invention provides a method for manufacturing a silica capillary column, comprises: attaching a double-bonded ligand to an inner wall of a silica capillary (step 1); manufacturing a polymerization mixture by dissolving a monomer mixture including a functional monomer, a cross-linked monomer, a pore inducing substance with a molecular weight range of 4,000 to 40,000, and a polymerization initiator in a mixed solvent consisting of a hydrogen binding solvent and a a non-hydrogen binding solvent (step 2); filling the silica capillary with a manufactured polymerization mixture (step 3); polymerizing by sealing both ends of the silica capillary filled with the polymerization mixture and heating the silica capillary (step 4); and cleaning the silica capillary after completing the polymerization (step 5). According to the present invention, the silica capillary column where a macromolecule thin film is formed on an inner wall surface of silica tubing to have proper pores has excellent manufacture reproducibility, and the most excellent separation efficiency is shown with respect to separation analysis of a proteomic sample (protein hydrolysis product) in a field of capillary electrochromatography (CEC) up to the present time. Furthermore, since a thin film, which is very easily be cleaned, is generated, the silica capillary column can be used as a high efficiency proteomic CEC column which can be produced in large quantities. |