| 摘要 |
PROBLEM TO BE SOLVED: To provide a colorimetric analysis method that couples sensor design with image processing to enable test results obtained by paper-based sensors to be automatically evaluated.SOLUTION: A robust colorimetric analysis processing method for paper based sensors, comprises: using a camera and taking a picture of a colorimetric paper sensor 100 for colorimetric reactions; identifying one color channel in which both a dye and reference color have near zero absorption and wherein the color channels in which both the dye and the reference color have absorption; identifying the known absorption ratio between the dye and the reference color in each color channel; normalizing each absorption channel by the near zero absorption channel to remove spatial variation; calibrating a test area reading with the reference color and a substrate white in the composite image; and reporting the concentration of a test substance analyte based on the calibrated reading in each test area.SELECTED DRAWING: Figure 3 |
