TYROSINE HYDROXYLASE VARIANTS AND METHODS OF USE THEREOF
摘要
The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.
申请公布号
WO2016049364(A2)
申请公布日期
2016.03.31
申请号
WO2015US52040
申请日期
2015.09.24
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;MARTIN, VINCENT J.J.;NARCROSS, LAUREN
发明人
MARTIN, VINCENT J.J.;NARCROSS, LAUREN;DUEBER, JOHN E.;DELOACHE, WILLIAM C.;RUSS, ZACHARY N.