Yeast strains

摘要

Several mutated strains of Scheffersomyces stipitis are generated by repetitive culturing of the parent strain on two types of concentrated hydrolyzates and with ethanol-challenged xylose-fed continuous culture. Isolates collected from various enriched populations are screened and ranked based on relative xylose uptake rate and ethanol yield. Ranking on hydrolyzates with and without nutritional supplementation is used to identify those isolates with best performance across diverse conditions.

主权项

1. An isolated Scheffersomyces stipitis strain capable of producing ethanol from glucose and xylose wherein said isolated S. stipitis strain can produce at least 40 g/L ethanol when cultured on non-detoxified lignocellulosic hydrolyzate, wherein said isolated S. stipitis strain has a reduced diauxic lag during sugar transition from glucose to xylose when cultured on said non-detoxified lignocellulosic hydrolyzate compared to the diauxic lag of S. stipitis ARS Culture Collection accession number NRRL Y-7124 when cultured in said non-detoxified lignocellulosic hydrolysate, and wherein said isolated S. stipitis strain is selected from the group consisting of S. stipitis having ARS patent deposit accession numbers NRRL Y-50871, Y-50859, NRRL Y-50862, Y-50864, and Y-50861.