Microfluidic cytochemical staining system

摘要

The present invention provides devices and associated methods to facilitate multiplexed staining on a slide. The use of multiplexed staining on a single slide allows for more accurate disease diagnosis while also conserving sample. A PDMS based cytology overlay was created that is capable of coupling on-chip morphological, cytochemical, and immunological staining. Morphological and cytochemical staining of blood smears, along with immunostaining of lymph node imprints, was carried out to demonstrate the application of this microfluidic cytology chip (μCC) device.

主权项

1. A microfluidic overlay device comprising:
a first substantially planar and substantially rectangular surface comprising:
a plurality of first ports adapted to receive a fluid, the ports spaced adjacent to one edge of the first surface; anda plurality of channels extending from the first ports, wherein the number of channels corresponds to the number of first ports; and a second surface opposite the first surface, the second surface comprising:
a plurality of channels linearly extending in parallel across the second surface; anda plurality of second ports adapted to facilitate egress of the fluid, wherein the second ports are in fluid communication with the second surface channels; and conduits, wherein each conduit provides fluid communication between a single first surface channel and a single second surface channel and the number of conduits corresponds to the number of channels on the first surface.