| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for effectively removing an interfering substance in measuring an extremely low density Phe in blood by a sensor; and to provide a method for measuring the Phe density in the blood with high precision. <P>SOLUTION: This method for measuring a phenylalanine or alanine density in blood using a Phe or Ala sensor that includes an electrode system having an action electrode where phenylalanine dehydrogenase or alanine dehydrogenase, and diaphorase are immobilized, and a counter electrode, includes the following (1)-(6) steps: (1) mixing the blood which is a test object with reagent solution including coenzyme and an electronic mediator to prepare measurement solution, (2) supplying the electrode system with the measurement solution, (3) applying a positive potential to the action electrode of the electrode system to oxidize ascorbic acid and the electronic mediator included in the measurement solution, (4) finishing the application of the positive potential and performing a enzyme reaction for a prescribed time, (5) after the lapse of the prescribed time, applying a positive potential to the action electrode of the electrode system to measure a current value, and (6) calculating the phenylalanine or alanine density in the blood which is the test object, based on the measured current value or an integrated value thereof. <P>COPYRIGHT: (C)2012,JPO&INPIT |
