METHOD FOR CONSTRUCTING A SEQUENCING LIBRARY BASED ON A SINGLE-STRANDED DNA MOLECULE AND APPLICATION THEREOF

摘要

A method for constructing a sequencing library based on a single-stranded DNA molecule is provided comprising: (1) forming a poly (C) n tail at a 3'-terminus of the single-stranded DNA molecule, to obtain a single-stranded DNA molecule with the poly (C) n tail with n representing a number of base C, and n being an integer ranging from 5 to 30; (2) obtaining a double-stranded DNA molecule by using an extension primer based on the single-stranded DNA molecule with the poly (C) n tail, with the extension primer comprising a H (G) m unit at a 3'-terminus thereof, H being base A, base T or base C, m being a number of base G, and m being an integer ranging from 5 to 15; and (3) ligating an adapter to one terminus of the double-stranded DNA molecule remote from the H (G) m unit, and amplifying the resulting ligation product to obtain an amplification product forming the sequencing library.