Cysteine engineered antibodies comprising a free cysteine amino acid in the heavy chain or light chain are prepared by mutagenizing a nucleic acid sequence of a parent antibody and replacing one or more amino acid residues by cysteine to encode the cysteine engineered antibody; expressing the cysteine engineered antibody; and isolating the cysteine engineered antibody.
申请公布号
WO2016040856(A2)
申请公布日期
2016.03.17
申请号
WO2015US49771
申请日期
2015.09.11
申请人
GENENTECH, INC.;F. HOFFMANN-LA ROCHE AG
发明人
BHAKTA, SUNIL;ERICKSON, HANS;JUNUTULA, JAGATH R.;KOZAK, KATHERINE;OHRI, RACHANA;PILLOW, THOMAS