Autoluminescent phytosensor plants and uses thereof

摘要

Real-time monitoring of plant or environmental conditions is solved by Autoluminescent Phytosensor Plants (ALPS) disclosed herein that emit light in response to a specified stimulus or condition, which light emission is detected or measured by a sensor.

主权项

1. A transplastomic plant cell containing a LUX operon comprising LUX genes integrated within a plastidal genome therein,
wherein any or all of said LUX genes are separated by an intercistronic expression element (IEE) operably linked thereto, wherein expression of said LUX genes is enhanced by a heterologous translational leader sequence operably linked to one or more of said LUX genes, wherein said heterologous translational leader sequence is a T7g10 leader sequence, and wherein said transplastomic plant cell is selected from the group consisting of a species of petunia and a species of Nicotiana.