| 摘要 |
FIELD: medicine.SUBSTANCE: invention relates to medicine and a method for diagnosing tuberculosis comprising isolating peripheral blood mononuclear cells, cultured them in a culture medium, obtaining a sample of culture supernatant of mononuclear cells in peripheral blood and carried immunosorbent assay (ELISA), wherein prior to ELISA in negative control well bring nutrient medium for culturing cells in wells positive control bring serum of patients with tuberculosis, obviously containing anti-Mtb IgG and anti-Mtb IgA diluted 1:100 in ELISA is used as a sorbent solution antigens Mtb and determining optical density of sample culture supernatant of mononuclear cells from peripheral blood of subjects OD, negative control sample Kand a positive control sample K, and then determine critical value of ratio of optical density OD= K0.1 and when OD values> OPand K> OPestablish diagnosis of active tuberculosis, with OD values< OPand K> OPestablish absence of TB infection or latent TB infection, and for values of K< OPand any values OPresults are unreliable.EFFECT: invention improves efficiency of diagnosing TB, while expanding scope of method and for differential diagnosis of tuberculosis and latent infection.1 cl, 6 ex |
