METHOD OF PRODUCING MYOBLASTS, USE OF GINGIVAL BIOPSY MATERIAL, MYOBLASTS PREPARATION FOR TREATING PATHOLOGIES OF MUSCULAR TISSUE AND METHOD FOR PRODUCTION THEREOF

摘要

FIELD: medicine.SUBSTANCE: invention relates to medicine, particularly to biotechnology, and can be used for producing myoblasts of oral mucosa of an individual. That is ensured by biopsy of oral mucosa and treatment of biopsy material for washing, milling, enzymatic splitting gingival tissue proteolytic enzymes. Further, method includes culturing fibroblast-like cells recovered from disaggregated tissue on a suitable medium for producing culture of myoblasts. Method includes performing immunophenotypic testing of myoblast culture for immunophenotype and exclusion of viral and bacterial contamination.EFFECT: use of present method enables to obtain a culture of myoblasts from a readily available source - human oral mucosa.2 cl, 1 ex, 2 dwg