Method and composition for staining and sample processing

摘要

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents, one or more permeabilizing agents, and one or more fixing agents. The particle contrast agent composition can include Crystal Violet, New Methylene Blue, Saponin, and Gluteraldehyde.

主权项

1. A particle contrast agent composition for staining a blood fluid sample being imaged in an automated particle analysis system comprising:
at least one particle contrast agent selected from the group consisting of Crystal Violet, New Methylene Blue, Methyl Green, Eosin Y, and Safranin O; a permeabilizing agent including saponin present in amounts sufficient to result in concentrations between about 50 mg/L and about 750 mg/L under staining conditions; and a fixing agent including gluteraldehyde present in amounts sufficient to result in concentrations at or below 0.1% under staining conditions.