THE USE OF LAMBDA-GAM PROTEIN IN RIBOSOMAL DISPLAY TECHNOLOGY

摘要

Methods and systems for increasing the stability of a nucleic acid template that encodes a protein of interest in a cell free translation system or a ribosomal display reaction system are described. In some embodiments, the nucleic acid template is an RNA or mRNA. The stability of the RNA template is increased by adding the bacteriophage lambda protein Gam to the cell free extract used in the transalation system. The addition of Gam protein increases the longevity of the reaction system, thereby increasing the efficiency of the ribosomal display reaction system.

主权项

1. A ribosomal display reaction system comprising a mRNA encoding a protein of interest without an operable stop codon, a cell free translation system, and a concentration of λGam protein sufficient to stabilize the mRNA encoding the protein of interest.