Method of Isolating and Purifying Fusion Protein Comprising Factor VII

摘要

The present invention provides a method of isolating and purifying a fusion protein comprising factor VII, and more specifically relates to a method of isolating and purifying a fusion protein comprising factor VII and transferrin, to a high degree of purity. Because the present invention provides a method whereby a recombinant fusion protein comprising factor VII can be isolated and purified to a high degree of purity, the invention is useful in producing a pharmaceutical preparation comprising factor VII that can be used in situations in which copious bleeding occurs such as surgery.

主权项

1. A method of isolating and purifying a fusion protein comprising factor VII, the method comprising:
1) isolating and purifying a fusion protein comprising factor VII expressed in animal cells by affinity chromatography using a resin having a structure represented by Formula 1 as a stationary phase or mixed-mode chromatography using a ceramic fluoroapatite gel (Ca10(PO4)6F2) as a stationary phase; and 2) further isolating and purifying the fusion protein by anion exchange chromatography using Q-sepharose FF gel as a stationary phase: wherein the matrix is cross-linked agarose, and R is a factor VII binding protein serving as a ligand. 15