METHODS AND KIT FOR CHARACTERIZING THE MODIFIED BASE STATUS OF A TRANSCRIPTOME

摘要

This invention relates to a method of characterizing the modified base status of a transcriptome, which involves contacting a transcriptome comprising one or more modified bases with an antibody specific to the modified bases under conditions effective to bind the antibody to the modified bases; isolating, from the transcriptome, a pool of RNA transcripts to which the antibody binds; and identifying isolated RNA transcripts that are present in a higher abundance in the isolated pool relative to the transcriptome, where each of said isolated RNA transcripts that are present in a higher abundance in the isolated pool together characterize the modified base status of the transcriptome. Also disclosed are a method of diagnosis or prognosis of a disease, a method of determining the effect of a treatment on modified base levels in RNA, and a kit for characterizing the modified base status of a transcriptome.

主权项

1. A method of characterizing the modified base status of a transcriptome, said method comprising:
contacting a transcriptome comprising one or more modified bases with an antibody specific to the one or more modified bases under conditions effective to bind the antibody to the one or more modified bases; isolating, from the transcriptome, a pool of RNA transcripts to which the antibody binds; and identifying isolated RNA transcripts that are present in a higher abundance in the isolated pool relative to the transcriptome, wherein each of said isolated RNA transcripts that are present in a higher abundance in the isolated pool together characterize the modified base status of the transcriptome.