Compositions, comprising improved IL-12 genetic constructs and vaccines, immunotherapeutics and methods of using the same

摘要

Nucleic acid molecules and compositions comprising: a nucleic acid sequence that encodes IL-12 p35 subunit or a functional fragment thereof and/or a nucleic acid sequence that encodes IL-12 p40 subunit or a functional fragment thereof, are disclosed. The nucleic acid molecules and compositions further comprising a nucleic acid sequence that encodes an immunogen are also disclosed. Method of modulating immune response and methods of inducing an immune response against an immunogen are disclosed. Therapeutic and prophylactic vaccination methods are also disclosed.

主权项

1. A composition that comprises a) a nucleic acid sequence that encodes IL-12 p35 subunit and b) a nucleic acid sequence that encodes IL-12 p40 subunit, wherein
the nucleic acid sequence that encodes IL-12 p35 subunit is at least 98% homologous to SEQ ID NO:1 and encodes a protein at least 98% homologous to SEQ ID NO:2, and the nucleic acid sequence that encodes IL-12 p40 subunit is at least 98% homologous to SEQ ID NO:3 and encodes a protein at least 98% homologous to SEQ ID NO:4.