Isoprene synthase variants for improved production of isoprene

摘要

The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved specific productivity. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.

主权项

1. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to K134, K138, L143, I156, E159, F163, S166, H167, E170, K414, or Q421 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of K134P, K138C, L143F, L143V, I156G, E159G, E159Q, F163C, F163E, F163Q, F163V, F163Y, S166C, S166D, S166G, S166P, S166V, H167M, E170G, E170H, E170K, E170N, E170R, E170S, E170W, K414F, K414G, K414N, K414P, and Q421R, and wherein a host cell expressing the polypeptide has at least 50% more growth compared to the growth of a host cell expressing a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 under similar growth conditions.