Simian Subfamily B Adenoviruses SAdV-28, -27, -29, -32, -33, and -35 and Uses Thereof

摘要

A recombinant vector comprises simian adenovirus 28, simian adenovirus 27, simian adenovirus 32, simian adenovirus 33, and/or simian adenovirus 35 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses one or more simian adenovirus-28, -27, -32, -33, or -35 genes is also disclosed. Methods of using the vectors and cell lines are provided.

主权项

1. An adenovirus having a capsid comprising a capsid protein selected from the group consisting of:
(a) a hexon protein of SAdV-29, amino acids 1 to 954 of SEQ ID NO: 81; a hexon protein of SAdV-32, amino acids 1 to 955 of SEQ ID NO: 113; a hexon protein of SAdV-33, amino acids 1 to 951 of SEQ ID NO: 144; a hexon protein of SAdV-35, amino acids 1 to 956 of SEQ ID NO: 176; (b) a penton protein of SAdV-28, amino acids 1 to 582 of SEQ ID NO: 6; a penton protein of SAdV-27, amino acids 1 to 562 of SEQ ID NO: 44; a penton protein of SAdV-29, amino acids 1 to 576 of SEQ ID NO: 76; a penton protein of SAdV-32, amino acids 1 to 585 of SEQ ID NO: 108; a penton protein of SAdV-33, amino acids 1 to 571 of SEQ ID NO: 139; a penton protein of SAdV-35, amino acids 1 to 563 of SEQ ID NO: 171; and (c) a fiber protein of SAdV-28, amino acids 1 to 323 of SEQ ID NO: 21, a fiber protein of SAdV-27, amino acids 1 to 325 of SEQ ID NO: 59; a fiber protein of SAdV-29, amino acids 1 to 324 of SEQ ID NO: 91; a fiber protein of SAdV-32, amino acids 1 to 319 of SEQ ID NO: 123; a fiber protein of SAdV-33, amino acids 1 to 325 of SEQ ID NO: 154; a fiber protein of SAdV-35, amino acids 1 to 353 of SEQ ID NO: 185; said capsid encapsidating a heterologous nucleic acid comprising a gene operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.