A METHOD OF DETECTING PORCINE MARKERS FOR IDENTIFYING PORK CONTENT IN A SAMPLE

摘要

The present invention relates to a method of detecting porcine markers for identifying a pork content in a test sample, comprising the steps of: extracting deoxyribonucleic acid (DNA) from the sample; performing polymerase chain reaction (PCR) on the extracted DNA using two pairs of primers, including a first forward primer and a first reverse primer for amplifying a target porcine DNA sequence, and a second forward primer and a second reverse primer (as an internal control) for amplifying a target gene sequence; then subjecting the amplified sequence to electrophoresis to detect the presence of porcine markers in the sample; characterized in that: the first forward primer comprises SEQ ID NO:1, the first reverse primer comprises SEQ ID NO:2, the second forward primer comprises SEQ ID NO:3, and the second reverse primer comprises SEQ ID NO:4.