| 摘要 |
Known detection methods for foreign substances mark gene sequences of living cells and detect, in a spatially resolved manner, the translocation of the stably expressed marked fusion constructions within the recombinant cells under the influence of foreign substance. The detection method according to the invention utilizes the dynamic expression behavior of recombinant living cells and detects in an integral manner. The cell-inherent defense system is preferably used, wherein gene sequences ai-mi in recombinant cells (19) as a preferred means in a test kit for performing the detection method code for transporter proteins Ai comprising fluorescent marker proteins Mi as fusion constructs Ai-Mi. The cell-inherent defense system is activated under the influence of foreign substance. Transporter proteins Ai that transport the foreign substance out of the living cells (19) are expressed in an increased manner. Together with the transporter proteins Ai, the marker proteins Mi are also expressed in an increased manner, which is optically detected and enables corresponding conclusions about the foreign substance (03). The detection method is preferably implemented by means of an arrangement (04) of genetically modified aquatic organisms (06) or living cells (19) thereof, which are permanently exposed in a water-permeable habitat tank (05) in an aquatic system (01) in the vicinity of a technical installation (02). |
