SYNTHESIS AND USE OF ISOTOPICALLY-LABELLED GLYCANS

摘要

Isotopically-labelled glycans and their synthesis and use as internal standards in the analysis by mass spectrometry of glycan mixtures is described. The methods of synthesis described herein may be used conveniently to prepare libraries of heavy glycans for use in the qualitative and quantitative identification of glycans in natural samples.

主权项

1. A method for the synthesis of an isotopically-labelled glycan for use as a mass spectrometry internal standard, the method comprising:
acylating an oligosaccharide core structure with an isotopically-labelled acylating agent, wherein the oligosaccharide core structure is optionally protected with one or more protecting groups, to obtain an isotopically-labelled oligosaccharide core structure; and enzymatically derivatising the resultant isotopically-labelled oligosaccharide to obtain the isotopically-labelled glycan.