| 摘要 |
FIELD: biotechnology.SUBSTANCE: invention relates to a set of synthetic oligonucleotides for species identification of Bupleurum multinerve (Bupleurum multinerve DC.), comprising polymerase chain reaction with a fragment ITS2 of nuclear DNA. And for identification species-specific portions are used to create the forward, reverse primers and destroyed probe, where the forward primer is the DNA sequence with the following nucleotide structure: 5'-TATCCCGGCTCTCGCATCGA-3'; the reverse primer is the DNA sequence with the following nucleotide structure: 5'-GACGAGGCACGGGAGGTCAG-3', the destroyed probe DNA sequence with the following nucleotide composition (fluorescent label)-5'-CCGTGAACCATCGAGTTTTT-3'-(quencher).EFFECT: invention enables the species identification of Bupleurum multinerve during screening of plant raw materials.1 dwg, 1 ex |
